Effects of silicon, calcium and phosphorus concentrations on the growth, differentiation and mineralization of dental pulp cells in vitro

Date
2003
DOI
Authors
Abbassi, Farah
Version
OA Version
Citation
Abstract
Application of silicon contained materials, are gaining ground in medical and dental field. These materials are used as synthetic bone graft material. The exact mechanism and role of silicon still remains largely unknown. The objective of this in vitro research was to examine the effect of conditioned medium with the silicon (Si), calcium (Ca), and phosphorous (P) on cell attachment, proliferation, and odontogenesis activities. Human pulp tissue cultures were established by harvesting pulp tissue from third molars of young patients. Pulp tissue cells cultured in the medium supplemented with Si (120, 60 ppm) alone, and its combination (60 ppm) with Ca (15 ppm) and P (5 ppm) for different time intervals at 16 hours, 6, 7, 9 days. The regular medium was used as a control in this study. The culture medium was measured for pH variation, cell attachment efficiency and proliferation rate were examined by measuring the optical density of crystal violet dye at 590 nm wavelength. Alkaline phosphatase (ALP) activity of the culture was measured using the p-nitrophenol reaction. I[125] radioisotope was used for labeling the expression of osteocalcin (OC). pH change during incubation of conditioned medium showed gradual decline of pH over a period of time, but statistical analysis showed no significant differences among all four groups of pH variation. (p=0.8250). However, there were significant overall differences among various groups in the cell attachment efficiency (p=0.000154), cell proliferation rate at 6 days (p=0.000154), ALP activity at 7 days of culture (p= 1.699x10[-2], Dentin sialoprotein (DSP) at 7 days (p= 1.32x10[-5]), and Osteocalcin (OC) 0= 4.61x10[-9]). These results demonstrated the important role of Si in odontogenesis, and how the combination of the three ions can multiply odontogenesis. Future study of silicon, calcium, and phosphorous is recommended, to clarify the relationship between odontogenesis and these materials at the cellular level.
Description
PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.
Thesis (M.S.D.) -- Boston University, Henry M. Goldman School of Dental Medicine, 2003 (Endodontics).
Includes bibliography (leaves 87-97).
License
This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.