Local antibody (IgG2) synthesis and subgingival microflora in peri-implant tissues

Date
1999
DOI
Authors
Robledo, Patricia
Version
OA Version
Citation
Abstract
Patients with periodontal disease frequently exhibit higher levels of specific IgG2 antibodies in gingival crevicular fluid (GCF) than in serum. This observation suggests that there is a localized production of these antibodies in the periodontal tissues. To date, no studies have investigated the presence or absence of these antibodies in fluids collected from peri-implant sites. The aim of the present investigation was to determine the relationship between the subgingival microflora and the local antibody response around implants. Ten subjects with a total of 39 implants, 34 healthy and 5 failing participated in the study. Antibody levels were measured in serum, saliva, and crevicular fluid from healthy implants, failing implants, and healthy teeth. Plaque samples were collected from the same sites. The subgingival microbiota was identified by DNA-DNA hybridization, and the presence of IgG[1, 2, 3, 4] was determined using “checkerboard” immunoblotting. Comparisons of IgG2 levels against several microorganisms were made between failing and non-failing implants, and between GCF and serum. Comparisons of the microbial fora of failing and nonfailing implants was performed. Statistical significance was tested with two-sided Fisher’s exact test. Three out of 5 failing implants revealed elevated antibody production of the IgG2 type against P. gingivalis and P. intermedia in the GCF. There was an inverse correlation between presence of bacteria and presence of antibody at diseased sites. Four out of 34 healthy, and 3 out of 5 failing implants showed evidence of elevated local IgG2 response. These differences were statistically significant at P [less than or equal to] 0.05 level. These observations indicate that there might be a correlation between local antibody production in the GCF and failing implants.
Description
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Thesis (M.Sc.D)--Boston University, Henry M. Goldman School of Dental Medicine, 1999 (Oral Biology).
Includes bibliographical references: (leaves 96-108).
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This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.