Two-photon phosphorescence lifetime microscopy of retinal capillary plexus oxygenation in mice
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Published version
Date
2018-12
Authors
Şencan, İkbal
Esipova, Tatiana V.
Yaseen, Mohammad A.
Fu, Buyin
Boas, David A.
Vinogradov, Sergei A.
Shahidi, Mahnaz
Sakadžić, Sava
Version
OA Version
Published version
Citation
İkbal Şencan, Tatiana V Esipova, Mohammad A Yaseen, Buyin Fu, David A Boas, Sergei A Vinogradov, Mahnaz Shahidi, Sava Sakadžić. 2018. "Two-photon phosphorescence lifetime microscopy of retinal capillary plexus oxygenation in mice.." J Biomed Opt, Volume 23, Issue 12, pp. 1 - 9. https://doi.org/10.1117/1.JBO.23.12.126501
Abstract
Impaired oxygen delivery and/or consumption in the retinal tissue underlies the pathophysiology of many retinal diseases. However, the essential tools for measuring oxygen concentration in retinal capillaries and studying oxygen transport to retinal tissue are still lacking. We show that two-photon phosphorescence lifetime microscopy can be used to map absolute partial pressures of oxygen (pO2) in the retinal capillary plexus. Measurements were performed at various retinal depths in anesthetized mice under systemic normoxic and hyperoxic conditions. We used a newly developed two-photon phosphorescent oxygen probe, based on a two-photon absorbing platinum tetraphthalimidoporphyrin, and commercially available optics without correction for optical aberrations of the eye. The transverse and axial distances within the tissue volume were calibrated using a model of the eye's optical system. We believe this is the first demonstration of in vivo depth-resolved imaging of pO2 in retinal capillaries. Application of this method has the potential to advance our understanding of oxygen delivery on the microvascular scale and help elucidate mechanisms underlying various retinal diseases.
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© The Authors. Published by SPIE and CLP under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI: 10.1117/1.JBO.23.12.126501.