Quantitative proteomics of gingival crevicular fluid and saliva from patients with periodontal disease

Date
2010
DOI
Authors
Carneiro, Leandro Goncalez
Version
OA Version
Citation
Abstract
Periodontal disease is a bacterial infection associated with a chronic inflammatory response, which affects tooth supporting structures. Manifestations of periodontal disease are gingival inflammation, alveolar bone loss, tooth mobility and in severe cases tooth loss, Such changes can lead to increased levels of serum related components in the oral cavity via gingival crevicular fluid (GCF). New components from the inflammatory reaction and bone resorption may concomitantly appear. To analyze these changes, GCF and whole saliva (WS) were collected from 30 subjects with moderate to severe periodontal disease and also from 30 subjects with no periodontal disease. GCF samples were collected with Periopaper strips (OrafIow, Plainview, NY) and both GCF and stimulated WS were collected from all subjects. The objectives of this study were first to establish the proteome of GCF from healthy sites. The second objective was to evaluate and determine the quantitative proteome of whole saliva supernatant (WSS) and GCF from healthy patients versus patients with periodontitis using LC-ESI-MS/MS technology coupled with isotope labeling. Local inflammatory response proteins were of particular interest, as they may reflect disease remission or exacerbation. The third objective was to validate the MS results with ELISA. [TRUNCATED]
Description
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Dissertation (DScD) --Boston University, Henry M. Goldman School of Dental Medicine, 2010 (Department of Periodontology and Oral Biology).
Includes bibliographic references: leaves 196-210.
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This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.