Nanosecond-resolution photothermal dynamic imaging via MHZ digitization and match filtering
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Published version
Date
2021-12-07
Authors
Yin, Jiaze
Lan, Lu
Zhang, Yi
Ni, Hongli
Tan, Yuying
Zhang, Meng
Bai, Yeran
Cheng, Ji-Xin
Version
Published version
OA Version
Citation
J. Yin, L. Lan, Y. Zhang, H. Ni, Y. Tan, M. Zhang, Y. Bai, J.-.X. Cheng. 2021. "Nanosecond-resolution photothermal dynamic imaging via MHZ digitization and match filtering.." Nat Commun, Volume 12, Issue 1, pp. 7097 - ?. https://doi.org/10.1038/s41467-021-27362-w
Abstract
Photothermal microscopy has enabled highly sensitive label-free imaging of absorbers, from metallic nanoparticles to chemical bonds. Photothermal signals are conventionally detected via modulation of excitation beam and demodulation of probe beam using lock-in amplifier. While convenient, the wealth of thermal dynamics is not revealed. Here, we present a lock-in free, mid-infrared photothermal dynamic imaging (PDI) system by MHz digitization and match filtering at harmonics of modulation frequency. Thermal-dynamic information is acquired at nanosecond resolution within single pulse excitation. Our method not only increases the imaging speed by two orders of magnitude but also obtains four-fold enhancement of signal-to-noise ratio over lock-in counterpart, enabling high-throughput metabolism analysis at single-cell level. Moreover, by harnessing the thermal decay difference between water and biomolecules, water background is effectively separated in mid-infrared PDI of living cells. This ability to nondestructively probe chemically specific photothermal dynamics offers a valuable tool to characterize biological and material specimens.
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© The Author(s) 2021. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.