Expression of connective tissue growth factor in gingival epithelial cells and fibroblasts
Date
2007
DOI
Authors
Xydas, Cristina Elena
Version
OA Version
Citation
Abstract
Introduction: Connective tissue growth factor (CTGF/CCN2) is expressed by human fibroblasts in response to TGFβ1 and stimulates the synthesis of the extracellular matrix (ECM). It has been implicated in etiology of fibrosis lesions throughout the body including drug-induced gingival overgrowth. In addition to the connective tissues of gingiva, CTGF/CCN2 is also expressed in the epithelium in histological analyses. Therefore, this study aimed to investigate the expression and activity of CTGF/CCN2 in human gingival fibroblasts and epithelial cells.
Methods: Primary gingival epithelial cells and fibroblasts were obtained from healthy donors. Cells were treated with TGFβ1, LPA, TGFβ1+LPA and endothelin. Western blotting was used to assess the protein levels for both epithelial cells and fibroblasts. Real time PCR was conducted to determine the mRNA expression of CTGF/CCN2.
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Description
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Thesis (MSD)--Boston University, Goldman School of Dental Medicine, 2007 (Dept. of Periodontology and Oral Biology).
Includes bibliographical references: leaves 65-74.
Thesis (MSD)--Boston University, Goldman School of Dental Medicine, 2007 (Dept. of Periodontology and Oral Biology).
Includes bibliographical references: leaves 65-74.
License
This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.