Early plasma cell infiltration in double mutant Akita NCF1 mice
Date
2012
DOI
Authors
Sama, Kishore Reddy
Version
OA Version
Citation
Abstract
Diabetes mellitus is a heterogenous group of metabolic disorders characterized by hyperglycemia due to insulin deficiency or its diminished effectiveness. Complete absence or low levels of superoxide production characterize chronic granulomatous disease by phagocytes (neutrophils, monocytes and eosinophils). The most common form of autosomal recessive CGD is caused by mutations in the gene p47 phox. Crossing Akita with Ncf1 produced heterozygous mice with both Akita and Ncf1 gene. A significant increase in the lung weight when compared to the wild type (WT) or the Ncf1 alone was observed. Microscopic examination of Akita/Ncfl lungs shows pulmonary inflammation with severe destruction of the lung tissue. Airways were completely obliterated with extensive inflammatory cell infiltrate. There was an increase in extracellular inflammatory exudates characterized by pale eosinophilic homogenous material along with areas of fibrosis.
Based on previous evidence, we hypothesized that there is an increase in plasma cells infiltration in the perivascular area of the lung tissues in a 6 to 8 week old double mutant knockout mice (Akita and Superoxide deficient Ncf1 mice). To determine the role of plasma cells in the 6 to 8 week old double mutant point mutation in the insulin gene) and the Ncf1 mice (a mutant p47 phox, a key element of the superoxide-generating NADPH oxidase found in leukocytes). Microscopic examination of Akita/Ncf1 mice showed perivascular mononuclear cellular infiltration in the 6-8 week old mouse. Peribronchiolar mononuclear cellular infiltrate is also seen but to a lesser extent when compared to the perivascular area. Immunohistochemistry analysis identified positive plasma cells.
In conclusion we have maintained an Akita/Ncf1 mouse model, which is diabetic and does not produce superoxide. Some amount of superoxide may be necessary in preventing lung damage and overproduction of superoxide in diabetes may contribute to tissue damage. Furthermore, the increased infiltration of plasma cells function in perivascular and peribrochiolar area, and the type of antibodies produced by them, needs to be explored.
Description
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Dissertation (MSD) --Boston University, Henry M. Goldman School of Dental Medicine, 2012 (Department of Periodontology and Oral Biology).
Includes bibliographic references: leaves 76-83.
Dissertation (MSD) --Boston University, Henry M. Goldman School of Dental Medicine, 2012 (Department of Periodontology and Oral Biology).
Includes bibliographic references: leaves 76-83.
License
This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.