Alpha V integrins on podocytes have a protective role during Adriamycin-induced glomerular injury in murine models
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Citation
Abstract
Integrins are an important class of cell-surface proteins that mediate extracellular-matrix adhesion. Our interest is in the role of integrins during glomerular disease. In this study, we investigate the role of alphaV integrins on podocytes in the repair response after Adriamycin-induced glomerular injury in adult mice. We hypothesize that alphaV integrins have a protective function during injury, and can be used as a therapeutic target to treat kidney diseases such as focal segmental glomerulosclerosis. To test our hypothesis, we conditionally deleted the ItgaV gene in podocytes of adult mice, using a podocyte-specific, tamoxifen-inducible Cre-loxP system. We successfully generated viable mice with the inactivated ItgaV gene. Prior to Adriamycin-induced injury, knockout mice were non-proteinuric and had intact glomerular filtration barriers, although glomerular capillaries were segmentally dilated. After Adriamycin-induced injury, we observed massive proteinuria in knockout compared to control mice, indicating the role of alphaV integrins in response to injury.
Description
2024
License
Attribution-NonCommercial-NoDerivatives 4.0 International