Molecular characterization and functional properties of human salivary mucins MG1 and MG2
Date
1999
DOI
Authors
Liu, Bing
Version
OA Version
Citation
Abstract
Nine human mucin genes have been identified to date and these have been named
MUCl-4 , MUC5AC, MUC5B and MUC6-8 . Human submandibular/sublingual gland
secretions contain both a high molecular weight mucin, MG 1 and a low molecular weight
mucin, MG2. The MUC5B gene product is a major component of MG 1 and MUC7 is a
single copy gene encoding MG2. MG 1 has been suggested to consist of a mixture of
mucin gene products and a major goal of this study was to identify other mucin genes
comprising MG 1. Polyclonal antibodies against deglycosylated MG 1 were used to screen
human submandibular and sublingual gland cDNA libraries and, after screening 200,000
pfus, no clones corresponding to previously unidentified mucins were found in either
library. Since previous Northern blotting studies indicated the presence of small
quantities of MUC4 mRNAs in submandibular gland, both libraries were screened with a
MUC4 DNA probe. Two clones containing MUC4 tandem repeats were isolated from the
submandibular gland library. A recombinant MUC4 protein was expressed in Escherichia
coli and this protein was found to react with polyclonal antibodies against deglycosylated
MG 1. These studies show conclusively that MG 1 contains both MUC5B and MUC4 gene
products suggesting that each mucin may perform distinct functions in the oral cavity. [TRUNCATED]
Description
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Thesis (D.Sc.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 1999 (Periodontology and Oral Biology).
Includes bibliographical references (leaves 176-194).
Thesis (D.Sc.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 1999 (Periodontology and Oral Biology).
Includes bibliographical references (leaves 176-194).
License
This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.