The development of a new bioassay system to assess the ability of drugs to accelerate tooth eruption
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Abstract
The purpose of this study was to design a bioassay procedure by means of which it would be possible to
rapidly screen drugs to determine their ability to
accelerate tooth eruption as well as their undesirable
side effects.
The rat maxillary 3rd molar was utilized as the
assay parameter for measuring acceleration in tooth
eruption.
The method developed utilizes female rats (Charles
River Breeding Lab), 26 days of age. Test drugs are
injected twice; when the rats are 26 and 27 days of age;
and the rats are sacrificed at 30 days of age. Heads
are removed and fixed in 95% ethernol for 12 hours.
The upper dental arches are examined under a disecting
microscope (lOOX) and the extent of eruption scored.
The extent of tooth eruption was determined by comparing 3rd molars to a standardized scoring chart.
The scoring chart depicts eleven progressive stages in
the eruption of the maxillary right and left 3rd molars.
This chart also contains a number or grade for each of
the eleven stages (from O, for no eruption, to 10 for
full eruption).
In control rats there is little or no eruption of
the 3rd molars at 30 days of age. Third molar eruption
was enhanced more than two times with a 300 ug/kg dose ... [TRUNCATED]
Description
Thesis (M.Sc.D.)--Boston University. Henry M. Goldman School of Graduate Dentistry, 1970 (Orthodontics)
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This work is being made available in OpenBU by permission of its author, and is available for research purposes only. All rights are reserved to the author.