Actinobacillus actinomycetemcomitans : its effect on chemotaxis and superoxide production on human neutrophils
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Abstract
Actinobacillus actinomycetemcomitans is believed to be the key player in the pathogenesis of localized aggressive periodontitis (LAP). To observe its effect on chemotaxis and superoxide production on human neutrophils, we primed human neutrophils with lipopolysaccharide (LPS). Primed cells were then stimulated with bacterial product analog, formyl-methionyl-leucyl-phenylalanine (fMLP). These cells were incubated for interaction (15-30 minutes). After 15 minutes, primed cells showed increased chemotactic attraction towards the bacterial product and increased production of superoxide. When compared with other bacteria species, the difference was not that significant, but superoxide production was slightly higher. Chemotactic activities were comparable. The increased level of superoxide may in part explain the detrimental effects that localized aggressive periodontitis have on the supporting periodontium around the affected area. Extravasates of these numerous superoxides are speculated to deteriorate the attachment apparatus and surrounding tissues.
Description
Thesis (MSD)--Boston University, Goldman School of Dental Medicine, 2007 (Dept. of Periodontology and Oral Biology).
Includes bibliographical references: leaves 37-43.
Includes bibliographical references: leaves 37-43.
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This work is being made available in OpenBU by permission of its author, and is available for research purposes only. All rights are reserved to the author.