Scanning electron microscopy of sulcular epithelium of Macaca Fascicularis
Date
1976
DOI
Authors
Kaplan, Gerald B.
Version
OA Version
Citation
Abstract
This investigation was concerned with the examination of the surface topography of sulular and col epithelia; their alteration with plaque and calculus accrual and the aberrations induced in epithelial surface morphology by inflammation. Five macaca fascicularis monkeys were utilized. Their teeth and marginal periodontal areas were clean ed 3 weeks pr ior to sacrifice at selected areas. The contra-lateral side was left untreated and served as control.
After sacrifice the jaws were dissected, segmented, fixed in 2% gluteraldehyde and subsequently demineralized, thus removing all enamel and giving access to the sulcular epithelium and col epithelium. The specimens were prepared for Scanning Electron Microscopy (SEM) through dehydration and critical point drying follovving which a layer of 200AO gold/palladium was deposited. After SEM observation the same specimens were processed for histologic examination employing standardized techniques. In this way direct correlation could be made between surface characteristics and histopathologic features.
SEMicrographs revealed micro-ulceration in the epithelium of periodontally involved teeth. This was confirmed histologically, which demonstrated ulcerative, erosive and disruptive epithelial changes and topical localization of plaque and calculus.
Description
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Thesis (M.Sc.D.)--Boston University, School of Graduate Dentistry, 1976.
Includes bibliography.
Thesis (M.Sc.D.)--Boston University, School of Graduate Dentistry, 1976.
Includes bibliography.
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This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.