Passive immunization with P gingivalis-specific IgG efficiently controls subsequent infection with P gingivalis in mice
Date
2004
DOI
Authors
Gonzalez, Dario E.
Version
OA Version
Citation
Abstract
Objective: Porphyromonas gingivalis is the principal organism associated with adult progressive periodontal disease, and despite rigorous clinical intervention strategies, periodontal disease continues to affect up to 30% of adults in the US. Recent studies suggest that immunization may be effective for the control of periodontitis; however, the nature of this protective antibody response is not well characterized. We adopted a passive antibody transfer strategy to begin to characterize the role of P. gingivalis-specific antibodies in protection from subsequent P. gingivalis challenge in a mouse subcutaneous chamber model.
Methods: Balb/C mice were immunized subcutaneously with 100 [microliters] of heat-killed P. gingivalis strain A7436 whole organism preparations, 3-times per week, for 3-weeks and the animals were exsanguinated. Serum was then applied to a high affinity protein G column, and total IgG was eluted and designated P. gingivalis- specific IgG (Pg-IgG). A commercially obtained Balb/C mouse IgG designated irrelevant-IgG (IRR-IgG) was used as an IgG control. Sterile stainless steel wire coils were surgically implanted in the subcutaneous tissue of the dorsolumbar region of 6-8 weeks old female BALB/c mice. At 24 hours prior to challenge, subcutaneous chambers were injected with a 100 [micrograms] of (Pg-IgG), or (IRR-IgG). The animals were then infected with 1x10 [superscript 9] C.F.U P. gingivalis strain A7436. Two additional groups of mice were challenged with a similar number of bacteria that were either opsonized with Pg-IgG or the irrelevant antibody. Chamber fluid samples were collected from all animals at l, 3, 7, 10, and 14 days after challenge and CFU/ml, total and viable inflammatory cell counts, as well as fluorescence phagocytosis assays were performed.
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Description
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Thesis (M.S.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 2004 (Periodontology and Oral Biology).
Includes bibliography (leaves 68-88).
Thesis (M.S.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 2004 (Periodontology and Oral Biology).
Includes bibliography (leaves 68-88).
License
This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.