Determining the effect of TLR-2 mutation on Porphyromonas gingivalis 381 elicited periodontal bone loss and atherosclerosis
Date
2005
DOI
Authors
Ostrer, Sofia
Version
OA Version
Citation
Abstract
The etiology and progression of periodontal disease is known to be mediated by dental bacterial plaque and the host response to this bacterial infection. While many pathogens have been identified in dental plaque, the presence of Porphyromonas gingivalis (P. gingivalis) is established to contribute to periodontal disease progression. It is increasingly becoming apparent that there is a link between active periodontal disease and systemic bacterial infections (i.e. atherosclerosis). Numerous studies have demonstrated that P. gingivalis is capable of invading and activating vascular epithelium. Whether local inflammation (as in periodontal disease) or systemic inflammation (as in atherosclerosis), the Toll Like Receptor (TLR) family of microbial pattern recognition receptors is known to mediate chronic inflammation (as seen in periodontitis and atherosclerosis). The purpose of this study was to investigate the impact of TLR-2 on the progression of P. gingivalis associated periodontal disease and atherosclerosis in mice.
The periodontal disease study involved thirty mice (BALB/c) divided into three groups of eight and one group of six. The control group, group l (n=8) was comprised of unaltered C57 mice. Group 2 (n=6) were orally challenged with P. gingivalis 381. Group 3 (n=8) were TLR-2 knockout mice. Group 4 (n=8) were TLR-2 knockout mice that were orally challenged with P. gingivalis 381. Mandibular bone height (CEJ to alveolar crest) measurements were taken in fourteen locations per mouse (seven on right and left) prior to testing and after harvesting. Results indicate that differences exist between group l & 3 (p=0.05) as well as between 2 & 4 (p=0.14). This investigation indicates that TLR-2 does in fact play a role in mediating periodontal bone loss in C57 mice.
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Thesis (MSD)--Boston University, Henry M. Goldman School of Dental Medicine, 2005 (Periodontology and Oral Biology).
Includes bibliographical references: leaves 49-59.
Thesis (MSD)--Boston University, Henry M. Goldman School of Dental Medicine, 2005 (Periodontology and Oral Biology).
Includes bibliographical references: leaves 49-59.
License
This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.