Exposure to periodontal pathogen porphyromonas gingivalis induces activation of acquired immune response reducing reparative bone formation
Date
2010
DOI
Authors
Ortiz, Javier J.
Version
OA Version
Citation
Abstract
Osteoimmunology is the interaction between osteology and immunology. There are many inflammatory diseases that activate the immune response and affect bone coupling. The purpose of this study is to investigate whether the activation of the acquired immune response increases uncoupling of bone formation in response the periodontal pathogen P. gingivalis. P. gingivalis was injected on the calvarial bone of mice with or without the prior immunization against the bacteria. Specimens were evaluated on day 5 and 8 post inoculation at the scalp and suture to determine the number of osteoclasts and the number of bone lining cells adjacent to the areas. The results show that immunization increases bone resorption, reduces bone formation and reduces coupling induced by P. gingivalis. The number of osteoclasts was higher in the immunized samples both on day 5 and day 8. Also the bone lining cell density was significantly reduced in the immunized group when compared to the non immunized group on both days. In conclusion we report that the inoculation with P. gingivalis to the scalp showed that on the suture and scalp area of the specimens that the immunized group had fewer bone lining cells on day 5 and day 8 compared to controls. Results show that the cytokines resulting from both the innate and acquired immune systems induce osteoblasts apoptosis. Bone lining cells may play an important role in reparative bone formation. When there is a strong host response to a bacterial infection, and this response is prolonged, the acquired immune response may cause apoptosis of these bone lining cells and reduce coupling.
Description
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Thesis (MSD) --Boston University, Goldman School of Dental Medicine, 2010 (Department of Periodontology and Oral Biology).
Includes bibliographic references: leaves 36-46.
Thesis (MSD) --Boston University, Goldman School of Dental Medicine, 2010 (Department of Periodontology and Oral Biology).
Includes bibliographic references: leaves 36-46.
License
This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.