CRISPR/Cas9 knockout to elucidate the role of Gpr183 in murine hematopoietic stem cell aging
Embargo Date
2027-03-05
OA Version
Citation
Abstract
Hematopoietic stem cell (HSC) aging is an ongoing field of research that investigates the contribution of HSCs to health decline in the elderly population. The aim of this study was to investigate the role of G protein-coupled receptor 183 (Gpr183), a gene that plays a role in lymphocyte migration and maturation, during HSC aging. Previous studies have found that Gpr183 is consistently upregulated in aged murine HSC, but it is unclear whether the gene has a functional role in aging. Mice transplanted with CRISPR/Cas9-based Gpr183 knockout aged HSCs were studied and the percentage of myelocytes and lymphocytes in the peripheral blood derived from the donor cells were analyzed. Results from flow cytometry showed a significant increase in myelocytes and decrease in lymphocytes compared to the control, suggesting that Gpr183 plays a crucial role in maintaining a balance between myelocytes and lymphocytes differentiated from HSCs. This study elucidated the direct role of Gpr183 in HSCs aging and offered valuable insights into the potential development of Gpr183 as a therapeutic target.
Description
2024