High throughput screening system for engineered cardiac tissues
Files
Date
2023
Version
Published version
OA Version
Citation
M.S. Ma, S. Sundaram, L. Lou, A. Agarwal, C.S. Chen, T.G. Bifano. 2023. "High throughput screening system for engineered cardiac tissues." Frontiers in Bioengineering and Biotechnology, Volume 11, pp.1177688-. https://doi.org/10.3389/fbioe.2023.1177688
Abstract
INTRODUCTION: Three dimensional engineered cardiac tissues (3D ECTs) have become indispensable as in vitro models to assess drug cardiotoxicity, a leading cause of failure in pharmaceutical development. A current bottleneck is the relatively low throughput of assays that measure spontaneous contractile forces exerted by millimeter-scale ECTs typically recorded through precise optical measurement of deflection of the polymer scaffolds that support them. The required resolution and speed limit the field of view to at most a few ECTs at a time using conventional imaging. METHODS: To balance the inherent tradeoff among imaging resolution, field of view and speed, an innovative mosaic imaging system was designed, built, and validated to sense contractile force of 3D ECTs seeded on a 96-well plate. RESULTS: The system performance was validated through real-time, parallel contractile force monitoring for up to 3 weeks. Pilot drug testing was conducted using isoproterenol. DISCUSSION: The described tool increases contractile force sensing throughput to 96 samples per measurement; significantly reduces cost, time and labor needed for preclinical cardiotoxicity assay using 3D ECT. More broadly, our mosaicking approach is a general way to scale up image-based screening in multi-well formats.
Description
License
© 2023 Ma, Sundaram, Lou, Agarwal, Chen and Bifano. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.