Biophysical changes in cell membranes during myogenesis 'in vitro'
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Abstract
The differentiation of chick embryo myoblasts in vitro was
studied using electron paramagnetic resonance (EPR) and dielectric
relaxation. Cell membranes of myoblasts in rotation culture for 72
hours were examined with three nitroxystearate (NSA) spin labels
which insert at increasingly greater depths into the lipid bilayer
(5-NSA < 12-NSA < 16-NSA). Changes in membrane order during myogenic
differentiation in vitro were seen. The spin label which
inserts near the bilayer surface (5-NSA) showed two peaks of
membrane order: one at 15 hours (associated with gyratory aggregation
and recovery of the myoblasts from dissociation) and one at 38
hours (associated with prostaglandin binding and increased cell ... [TRUNCATED]
Description
Thesis (Ph.D.)--Boston University
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PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.