The role of PDGF-A-fibronectin interactions in the directed migration of the mesendoderm during gastrulation
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Abstract
Platelet-derived growth factor-A (PDGF-A) is essential for processes involving
cell motility throughout embryonic development. Here we examined the
underlying basis of its role as a guidance cue for the directed migration of
anterior mesendoderm cells during a period of cell reorganizations called
gastrulation. In Xenopus laevis embryos, these cells migrate across a fibronectinrich
extracellular matrix (ECM) that lines the blastocoel roof (BCR). We tested
the hypothesis that PDGF-A mRNA is in a graded pattern across the BCR that is
modulated by mesendoderm cells as they move across it. Quantitative reverse
transcriptase polymerase chain reactions were performed on BCR tissue
sections. Throughout gastrulation, the overall levels of PDGF-A mRNA
increased, and at all developmental stages examined, the same pattern of
PDGF-A mRNA was identified. PDGF-A mRNA levels were lowest at the
blastopore lip and although higher levels were detected across the rest of the ... [TRUNCATED]
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Thesis (Ph.D.)--Boston University
PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.
PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.