Altered lymphocyte activation and macrophage apoptosis in diabetic mice
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Abstract
The purpose of this study was to explore whether lymphocyte activation and
mononuclear cell apoptosis is dis-regulated by a diabetic situation which results in altered responses to bacterial infection. Based on other investigator's results NO production by
macrophages is increased in diabetic mice therefore, the effects of NO on these processes
are also examined.
Diabetic (db-/db-) and background matched control (db+/db-) mice were injected with or
without LPS. An iNOS inhibitor (AMT) was used in selected experiments. Activation of
spleen lymphocytes and apoptosis of the mononuclear cells was examined using flow
cytometry and ELISA.
The results suggest that diabetes could affect lymphocyte activation and macrophage
apoptosis. Nitric oxide may play a role in CD69 expression by diabetic T lymphocytes
and have an opposite effect on B lymphocytes. These findings support the hypothesis that
the kinetics and magnitude of the host response in diabetic and normal mice are different,
which leads to an altered inflammatory course during the microbial infection.
Description
Thesis (M.S.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 2004 (Periodontology).
Includes bibliography (leaves 41-50).
Includes bibliography (leaves 41-50).
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This work is being made available in OpenBU by permission of its author, and is available for research purposes only. All rights are reserved to the author.