Three-photon imaging of mouse brain structure and function through the intact skull
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Date
2018-10
Authors
Wang, Tianyu
Ouzounov, Dimitre G.
Wu, Chunyan
Horton, Nicholas G.
Zhang, Bin
Wu, Cheng-Hsun
Zhang, Yanping
Schnitzer, Mark J.
Xu, Chris
Version
Accepted manuscript
OA Version
Citation
T. Wang, D.G. Ouzounov, C. Wu, N.G. Horton, B. Zhang, C.-H. Wu, Y. Zhang, M.J. Schnitzer, C. Xu. 2018. "Three-photon imaging of mouse brain structure and function through the intact skull." Nature Methods, Volume 15, Issue 10, pp.789-792. https://doi.org/10.1038/s41592-018-0115-y
Abstract
Optical imaging through the intact mouse skull is challenging because of skull-induced aberrations and scattering. We found that three-photon excitation provided improved optical sectioning compared with that obtained with two-photon excitation, even when we used the same excitation wavelength and imaging system. Here we demonstrate three-photon imaging of vasculature through the adult mouse skull at >500-μm depth, as well as GCaMP6s calcium imaging over weeks in cortical layers 2/3 and 4 in awake mice, with 8.5 frames per second and a field of view spanning hundreds of micrometers.