Membrane-bound mucins in the oral environment: development of immunological assays to quantify MUC1
Date
2006
DOI
Authors
Amano, Koki
Version
OA Version
Citation
Abstract
MUC 1 and MUC4 are two membrane-bound mucins which are expressed on many
epithelial surfaces throughout the body including the oral cavity. The functions of these
mucins in normal ells diverse, ranging from physiological roles in cell growth, differentiation,
uterine embryo implantation, epithelial protection, bacterial attachment and signal
transduction. In malignant cells, these mucins assume pathological roles in the masking of
recognition sites for natural killer cells and promotion of tumor cell metastasis. Little is
known, however, concerning the functions of these mucins in the oral cavity and the aim of
this study was to develop a procedure to purify these mucins to homogeneity and determine
their concentration in a variety of biological samples.
The isolation of mucin proteins from cell culture medium proved problematic and
efforts were therefore focused on the development of an immunological assay to measure
MUC 1. Conditions for the assay were optimized and it was possible to demonstrate a linear
relationship between protein concentration and optical density in a sandwich ELISA. The
newly developed assay was then used to measure MUCl secretion in response to treatment of
oral epithelial cells with the periodontal pathogen, Porphyromonas gingivalis. [TRUNCATED]
Description
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Thesis -- Boston University Goldman School of Dental Medicine, 2006.
Thesis -- Boston University Goldman School of Dental Medicine, 2006.
License
This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.