In vivo analysis of lysyl oxidase deletion in mice, a pilot study
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Abstract
Lysyl Oxidase (LOX) is a key enzyme for the production of collagen cross-links in connective tissue development. This study investigated the phenotypic effects of mouse Lox deletion using Cre-recombinase technology driven by the promoter of the osteoblast specific hormone osteocalcin. 6 Female Ocn-cre-/-, Lox fl/fl; 6 female Ocn-cre+/-, Loxfl/fl; 6 male Ocn-cre-/-, Loxfl/fl, and 6 male Ocn-cre+/-, Loxfl/fl were sacrificed at 13-weeks for µCT and histological analysis. µCT data revealed statistically significant reduction in Bone Volume/Total Volume (BV/TV) (P<.003), Connective Tissue Density (ConnD) (P<.012), Bone Mineral Density (BMD) (P<.001), and Structural Model Index (SMI) (P<.004). Male 13-week old Ocn-cre+/-, Loxfl/fl mice showed no statistically significant differences in any parameters analyzed. Histological analysis showed decreases in the number of chondrocytes per column in the cre+/- female and male mice. The largest differences in number of chondrocytes per column were found in female cre+/- mice. Thus, osteoblast specific knockout of Lox appeared to show phenotypic affects more strongly in females rather than male mice. Further analysis into Lox knockout should be conducted.
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Attribution-NonCommercial-NoDerivatives 4.0 International