In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain
Date
2017-04
Authors
Ouzounov, Dimitre G.
Wang, Tianyu
Wang, Mengran
Feng, Danielle D.
Horton, Nicholas G.
Cruz-Hernández, Jean C.
Cheng, Yu-Ting
Reimer, Jacob
Tolias, Andreas S.
Nishimura, Nozomi
Version
Accepted manuscript
OA Version
Citation
D.G. Ouzounov, T. Wang, M. Wang, D.D. Feng, N.G. Horton, J.C. Cruz-Hernández, Y.-T. Cheng, J. Reimer, A.S. Tolias, N. Nishimura, C. Xu. 2017. "In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain." Nature Methods, Volume 14, Issue 4, pp.388-390. https://doi.org/10.1038/nmeth.4183
Abstract
High-resolution optical imaging is critical to understanding brain function. We demonstrate that three-photon microscopy at 1,300-nm excitation enables functional imaging of GCaMP6s-labeled neurons beyond the depth limit of two-photon microscopy. We record spontaneous activity from up to 150 neurons in the hippocampal stratum pyramidale at ∼1-mm depth within an intact mouse brain. Our method creates opportunities for noninvasive recording of neuronal activity with high spatial and temporal resolution deep within scattering brain tissues.