Differential effects of anesthetics on resting state functional connectivity in the mouse
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Date
2020-04
Authors
Xie, Hongyu
Chung, David Y.
Kura, Sreekanth
Sugimoto, Kazutaka
Aykan, Sanem A.
Wu, Yi
Sakadžić, Sava
Yaseen, Mohammad A.
Boas, David A.
Ayata, Cenk
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Hongyu Xie, David Y Chung, Sreekanth Kura, Kazutaka Sugimoto, Sanem A Aykan, Yi Wu, Sava Sakadžić, Mohammad A Yaseen, David A Boas, Cenk Ayata. 2020. "Differential effects of anesthetics on resting state functional connectivity in the mouse.." J Cereb Blood Flow Metab, Volume 40, Issue 4, pp. 875 - 884. https://doi.org/10.1177/0271678X19847123
Abstract
Blood oxygen level-dependent (BOLD) functional MRI (fMRI) is a standard approach to examine resting state functional connectivity (RSFC), but fMRI in animal models is challenging. Recently, functional optical intrinsic signal imaging-which relies on the same hemodynamic signal underlying BOLD fMRI-has been developed as a complementary approach to assess RSFC in mice. Since it is difficult to ensure that an animal is in a truly resting state while awake, RSFC measurements under anesthesia remain an important approach. Therefore, we systematically examined measures of RSFC using non-invasive, widefield optical intrinsic signal imaging under five different anesthetics in male C57BL/6J mice. We find excellent seed-based, global, and interhemispheric connectivity using tribromoethanol (Avertin) and ketamine-xylazine, comparable to results in the literature including awake animals. Urethane anesthesia yielded intermediate results, while chloral hydrate and isoflurane were both associated with poor RSFC. Furthermore, we found a correspondence between the strength of RSFC and the power of low-frequency hemodynamic fluctuations. In conclusion, Avertin and ketamine-xylazine provide robust and reproducible measures of RSFC in mice, whereas chloral hydrate and isoflurane do not.
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