Alanine aminotransferase assay biosensor platform using silicon nanowire field effect transistors
Files
Published version
Date
2023-03-01
Authors
Muratore, Katherine A.
Zhou, Dan
Du, Jiangang J.
Chlystek, John S.
Motesadi, Kasra
Larsen, Erik K.
Molgora, Brenda M.
Lee, Tetz C.
Pamarti, Sudhakar
Erramilli, Shyamsunder
Version
OA Version
Citation
K.A. Muratore, D. Zhou, J.J. Du, J.S. Chlystek, K. Motesadi, E.K. Larsen, B.M. Molgora, T.C. Lee, S. Pamarti, S. Erramilli, P. Mohanty. "Alanine aminotransferase assay biosensor platform using silicon nanowire field effect transistors" Communications Engineering, Volume 2, Issue 1. https://doi.org/10.1038/s44172-023-00057-4
Abstract
Frequent monitoring of serum alanine aminotransferase (ALT) activity is essential to prevent drug-induced liver injury (DILI). Current ALT assays are restricted to centralized clinical laboratories, making frequent patient monitoring logistically difficult. To address this, we demonstrated the capability of commercial foundry manufactured silicon nanowire field effect transistor (SiNW-FET) biosensors in a form factor that enables frequent near-patient monitoring. Here, we designed an ALT assay, by coupling the ALT-catalyzed production of pyruvate to the reduction of ferricyanide, enabling both spectrophotometric and electrical measurement of ALT activity. The two methods yield comparable ALT activity detection across a dynamic range wide enough to monitor patients at risk for DILI. This study demonstrates kinetic activity measurement of an endogenous enzyme using uncoupled SiNW-FETs, and commercial manufacturing of SiNW-FET sensor arrays for use in a portable biosensor platform.
Description
License
© The Author(s) 2023. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.