The biocompatibility of tungsten as a radiopacifier on root repair material
Date
2012
DOI
Authors
Mazzuca, Marci Christine
Version
OA Version
Citation
Abstract
The aim of this study was to investigate, on normal human fibroblast cells, the biocompatibility of an experimental root repair material containing tungsten as a radiopacifying agent. Tungsten heavy alloys are used in different industries, mainly because of their resistance to corrosion, temperature, and wear. Tungsten was chosen in this experiment as an alternative to bismuth oxide to improve the materials overall properties as well as improved radiopacity.
Methods: the root repair material chosen was Mineral Trioxide Aggregate. Its components were mixed and incubated 24 hours prior to testing in 24 wells plate. Normal adult human primary dermal fibroblasts were grown in fibroblast basal media supplemented with fibroblast growth kit components, to provide an ideal cell system to propagate dermal fibroblasts in low serum conditions. The cells were then seeded at a density of 1.5x10 superscript 5 cells in each well. Cells grown alone in the wells served as a control. Crystal violet dye was used to measure cell attachment efficiency and proliferation rate at 12 and 20 days. Cell attachment to conventional MTA with bismuth oxide and attachment to experimental MTA with tungsten was measured at 16hrs. Proliferation of cells was determined at 12 days and 20 days. Type 1 collagen expression was measured by ELISA assay at 12 and 20 days. The data was analyzed with t-test sample analysis.
Results: The cell attachment results showed the control group and the experimental group had equivalent attachment efficiencies (P=0.638). The null hypothesis could not be rejected. Attachment efficiency of the control group at 16hrs was 81% and the experimental group was 78%. Cell proliferation on the control group MTA increased l.44 fold at day 12 and on the experimental MTA increased 1.42 fold at day 12 (P=0.44). At day 20 the cell proliferation increased 1.72 fold on the control group MTA and increased 1.70 fold on the experimental group MTA (P=0.46). The null hypothesis could not be rejected at both time intervals. Type 1 collagen expression was detected at 12 and 20 day time intervals on both materials. The expression of Type 1 collagen on the control and experimental materials was equivalent at day 12 (P=0.34) and day 20 (P=0.57). The null hypothesis could not be rejected for both time intervals.
Conclusion: Tungsten is a good alternative as a radiopacifying agent in root repair material as it is biocompatible with human dermal fibroblast cell mode. Results from this study demonstrate that tungsten modified MTA has similar biocompatibility to conventional MTA and may be a promising substitute as a new radiopacifier of dental materials.
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Thesis (MSD) --Boston University, Henry M. Goldman School of Dental Medicine, 2012 (Department of Endodontics).
Includes bibliographic references: leaves 46-53.
Thesis (MSD) --Boston University, Henry M. Goldman School of Dental Medicine, 2012 (Department of Endodontics).
Includes bibliographic references: leaves 46-53.
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This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.