Impaired neutrophil chemotaxis in juvenile periodontitis
Date
1981
DOI
Authors
Owens, June Hayward
Version
OA Version
Citation
Abstract
The objective of this study was to evaluate polymorphonuclear (PMN) chemotaxis in patients with juvenile periodontitis (JP), their siblings and patients with other forms of periodontal disease. Patients were categorized into three groups after complete medical and dental histories, clinical and radiographic examinations to determine location and extent of plaque and other deposits, the degree of periodontal inflammation and alveolar bone resorption. Anaerobic bacterial sampling of normal and diseased sites was performed (before initial sealing procedures) to determine the predominant cultivatable flora according to patient availability. The groups and age ranges are as follows: JP, 11-20 years (10 patients); post-JP, 21-30 years (3 patients); and adult periodontitis-AP, 30 years and older (1 patient). All of the juvenile periodontitis group were of Black American or Portuguese (Cape Verdian) heritage. A large female:male ratio was observed (4:1) which is consistent with several recent investigations. Affected siblings as well as unaffected siblings are included in this study. Controls for this study were healthy, 18-30 year old laboratory personnel without periodontal disease. In 7 of 9 patients tested, the “typical” gram(-) anaerobic periodontosis flora was cultivated from diseased sites in high percentages of the total cultivatable flora.
PMN chemotaxis was evaluated prior to periodontal therapy, an average of 2-3 occasions, employing 51Cr-labeled PMN's and a double micropore filter Boyden chamber system. The responses of patient and control PMN’s were determined with zymosan activated serum as the chemoattractant and gelatin veronal buffer alone for random migration in quadruplicate chambers. After three hours incubation, chemotaxis was determined as the counts/minute of 51Cr in the lower filter corrected for the specific uptake of radioactivity of the neutrophils.
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Description
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Colored photographs included.
Thesis (M.Sc.D.)--Boston University, Henry M. Goldman School of Graduate Dentistry, 1981 (Oral biology)
Bibliography : leaves 121-140.
Colored photographs included.
Thesis (M.Sc.D.)--Boston University, Henry M. Goldman School of Graduate Dentistry, 1981 (Oral biology)
Bibliography : leaves 121-140.
License
This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.