Mechanisms for LPS induced fibroblast apoptosis
Date
2004
DOI
Authors
Alikhani, Mani
Version
OA Version
Citation
Abstract
Following Gram-negative bacterial infection there is a reduction in matrix producing
cells. The goal of the present study was to examine the apoptotic effects of
lipopolysaccharide (LPS) on fibroblastic cells and to investigate the role that the host
response plays in this reaction. This was accomplished in vivo by subcutaneou s
inoculation of LPS in wild type, TNFR 1-1-R2 -t- 1, TNFR 1-1- and TNFRT '- mice . The
direct effects of LPS on fibroblast apoptosis was studied in vitro with normal diploid
human fibroblasts. In vitro studies demonstrated that LPS did not induce apoptosis of
fibroblasts whereas TNF did. Histological studies indicated that LPS in vivo induced
apoptosis of fibroblasts. Similar results were obtained by subcutaneou s injection of
TNF-a in wild type mice. Histological studies on TNFR 1-1-R2-1- mice demonstrated
that LPS-induced fibroblast apoptosis was largely dependent on TNF-a. Fluorometric
studies demonstrated that LPS in vivo significantly increased caspase-8 and caspase-3
activity and by use of specific inhibitors, the activation of caspase-3 was shown to be
initiated by caspase-8. [TRUNCATED]
Description
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Thesis (D.Sc.)--Boston University; Henry Goldman School of Graduate Dentistry, 2004.
Includes bibliography (leaves 102-122).
Thesis (D.Sc.)--Boston University; Henry Goldman School of Graduate Dentistry, 2004.
Includes bibliography (leaves 102-122).
License
This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.