Measurement of oxidation-reduction potential in gastric and bronchial aspirates as a novel biomarker in the evaluation of children with known or suspected gastroesophageal reflux disease
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Abstract
INTRODUCTION: Gastroesophageal Reflux Disease (GERD) is a common, multifaceted, chronic disease that affects the esophagus. In chronic disease, a disproportionate amount of reactive oxygen species are released from dysregulated inflammatory cells, resulting in pathological inflammation. Measuring reactive oxygen species by oxidation-reduction potential may be a novel method for evaluating GERD.OBJECTIVES: This study aims to explore the relationship between the oxidation-reduction potential (ORP) of bronchial and gastric aspirates in patients with known or suspected gastroesophageal reflux or aspiration.
METHODS: Bronchial and gastric aspirates were collected from patients scheduled in the Gastrointestinal Procedural Unit (GPU) at Boston Children’s Hospital during endoscopy procedures from May 2022 to January 2024. ArrowDOX and Mettler Toledo probes were used to measure the ORP of each clinical sample. ORP values were measured in millivolts (mV). Statistical analysis included an assessment of the concordance of the measured ORP values recorded using different probes. Metadata was extracted from the electronic medical record to assess the relationship between ORP and the other clinical and epidemiology parameters.
RESULTS: Redox status between paired samples with the ArrowDOX probe were significant. Redox status between different aspirates and variables such as esophagitis, dysphagia, heartburn symptoms, aspiration, and eosinophil number did not differ significantly.
CONCLUSIONS: The measurement of redox status in gastric and bronchial fluids does not appear to significantly provide diagnostic or categorical information about a patient’s disease.
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2024