Genetic cloning of a protease and complete nucleotide sequence of a neuraminidase from streptococcus oralis C104
Date
1999
DOI
Authors
Almeida, Anna Galganny
Version
OA Version
Citation
Abstract
Streptococcus sanguis, Streptococcus oralis and Streptococcus mitis
are among the earliest colonizers of the tooth surface during
plaque formation. In addition, these species are also associated
with extraoral infections including infective endocarditis. A wide
range of proteolytic enzymes produced by S. oralis is believed to
play an essential role in plaque formation and systemic diseases.
Furthermore, neuraminidases, enzymes that cleave sialic acids
from carbohydrates and glycoproteins, are believed to play a role
in plaque formation and extraoral infections by oral streptococci.
In order to identify the gene(s) encoding the S. oralis
neuraminidase, a genomic library from S. oralis C104 was
constructed in Lambda ZAP II and screened with MUN (a
fluorescent neuraminidase substrate, 2'-(4-methylumbelliferyl)-aD-
N-acetylneuraminic acid) to detect plaques with neuraminidase
activity. Approximately 500,000 plaques were screened and 15
plaques with neuraminidase activity were obtained. E. coli
containing recombinant plasmid obtained by in mvo excision from
9 neuraminidase-positive plaques were subcultured and
expressed neuraminidase activity. [TRUNCATED]
Description
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Thesis (D.Sc.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 1999 (Pediatric Dentistry).
Includes bibliographical references (p. 81-109).
Thesis (D.Sc.D.)--Boston University, Henry M. Goldman School of Dental Medicine, 1999 (Pediatric Dentistry).
Includes bibliographical references (p. 81-109).
License
This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.