Generation of a purified iPSC-derived smooth muscle-like population for cell sheet engineering
Date
2019-09-10
Authors
Kwong, George
Marquez, Hector A.
Yang, Chian
Wong, Joyce Y.
Kotton, Darrell N.
Version
Published version
OA Version
Citation
G. Kwong, H.A. Marquez, C. Yang, J.Y. Wong, D.N. Kotton. 2019. "Generation of a Purified iPSC-Derived Smooth Muscle-like Population for Cell Sheet Engineering.." Stem Cell Reports, Volume 13, Issue 3, pp. 499 - 514. https://doi.org/10.1016/j.stemcr.2019.07.014
Abstract
Induced pluripotent stem cells (iPSCs) provide a potential source for the derivation of smooth muscle cells (SMCs); however, current approaches are limited by the production of heterogeneous cell types and a paucity of tools or markers for tracking and purifying candidate SMCs. Here, we develop murine and human iPSC lines carrying fluorochrome reporters (Acta2hrGFP and ACTA2eGFP, respectively) that identify Acta2+/ACTA2+ cells as they emerge in vitro in real time during iPSC-directed differentiation. We find that Acta2hrGFP+ and ACTA2eGFP+ cells can be sorted to purity and are enriched in markers characteristic of an immature or synthetic SMC. We characterize the resulting GFP+ populations through global transcriptomic profiling and functional studies, including the capacity to form engineered cell sheets. We conclude that these reporter lines allow for generation of sortable, live iPSC-derived Acta2+/ACTA2+ cells highly enriched in smooth muscle lineages for basic developmental studies, tissue engineering, or future clinical regenerative applications.
Description
License
© 2019 The Author(s). This article is available under the Creative Commons CC-BY-NC-ND license and permits non-commercial use of the work as published, without adaptation or alteration provided the work is fully attributed.