Instigation in TNF alpha promoter activity in response to LPS
OA Version
Citation
Abstract
LPS-induced activation of transcription factors has been shown to be a key regulator of tumor necrosis factor-α (TNF-α) production. TNF-α synthesis may be attenuated in immune cells exposed to phosphodiesterase (PDE) inhibition after challenge with various pro-inflammatory stimulants. The signaling mechanisms affected by PDE inhibition that led to down-regulation of TNF-α production are not well characterized in inflammatory cells. Activation of certain transcription factors has been found to reduce TNF-α-induced inflammation by downregulating TNF-α promoter activity. The pathways by which these activated transcription factors downregulate TNF-α promoter activity are largely unknown. The overall aim of this study was to determine the biological function of these factors in LPS/TNF mediated signaling.
Aim 1: to clone mouse or human TNF-α promoter DNA.
Aim 2: analyze TNF-a promoter activity