An adaptive optics module for deep tissue multiphoton imaging in vivo
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Date
2021-10
Version
Accepted manuscript
OA Version
Citation
C. Rodríguez, A. Chen, J.A. Rivera, M.A. Mohr, Y. Liang, R.G. Natan, W. Sun, D.E. Milkie, T.G. Bifano, X. Chen, N. Ji. 2021. "An adaptive optics module for deep tissue multiphoton imaging in vivo." Nature Methods, Volume 18, Issue 10, pp.1259-1264. https://doi.org/10.1038/s41592-021-01279-0
Abstract
Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at great depths in vivo.