Characterization of the lactate permease operon of Veillonellae Atypica PK 1910
Date
2002
DOI
Authors
Babae, Raed Ahmed
Version
OA Version
Citation
Abstract
Veillonellae have been extensively studied and have been found to have an unusual
metabolism. They do not metabolize carbohydrates such as glucose as an energy source
for growth, instead, they can grow well anaerobically in the presence of nonfermentable
substrates. It has been proposed that Vel/onellae play an important role in reducing the
cariogenic potential of plague bacteria. From a previous study, three ORFs were
identified in let permease operon of V. atypica PK 1910: ORFl (mdh) of 1.136 hp, ORF2
(lctP) of 1.641 bp, and ORF3 (hem) of 1.257 bp. In this study we focused on the
expression and regulation of lctP operon in V. atypica PK 1910 by environmental
conditions. The bacteria was grown anaerobically in Scaedler broth supplemented with
different acids: lactate, pyruvate, malate, formate, fumarate, and succinate. The growth
was significantly better with lactate or pyruvate containing media. RNA purification was
successful from the bacteria grown in different media in all different growth phases
(logarithmic phase, stationary phase, and overnight culture) examined. [TRUNCATED]
Description
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Thesis (D.Sc.D.)--Boston University Henry M. Goldman School of Dental Medicine, 2002 (Pediatric Dentistry).
Thesis (D.Sc.D.)--Boston University Henry M. Goldman School of Dental Medicine, 2002 (Pediatric Dentistry).
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This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.