Synthesis optimization of lipid-wrapped polymer nanoparticles
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Abstract
Nanoparticles (NPs) have become a robust drug delivery platform and are in extensive use in the pharmaceutical industry. Lipid-wrapped polymer NPs combine the advantages of liposomes and polymer NPs together and exclude some limitations of both. This thesis reports how to optimize the synthesis outcome of lipid-wrapped polymer NPs by changing the lipid/polymer ratio, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine(DOPS) composition in lipids and the choice of polymer materials. The in vitro stability of the synthesized NPs were tested in 1× PBS buffer in terms of size at different time points up to 7-day at room temperature. The lipid-wrapped polymer NPs were prepared by one-step nanoprecipitation and self-assembly aided by bath sonication for five minutes. We characterized NPs by measuring the hydrodynamic diameters and zeta potential through Dynamic Light Scattering (DLS), measuring the absorbance through Ultraviolet–visible spectroscopy (UV-Vis) and observing the morphology and size distributions through Scanning Electron Microscope (SEM). We find the surfactant DOPS ratio has a significant influence on size distribution. And NPs with different polymer materials are stable in 1 × PBS buffer within 7 days. There is a clear negative correlation between PDI and lipid/polymer ratio, which indicates the potential to control PDI by adjusting the lipid/polymer ratio. The result of this project has the potential use in precisely controlling the sizes and size distributions of drug-loaded NPs and scaling up the production in pharmaceutical manufacture.
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Attribution-NonCommercial-NoDerivatives 4.0 International