The effect of platelet-derived growth factor (PDGF) on human osteoblast migration and modulations by endotoxin and interleukin-1 B
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Abstract
Lesions of endodontic origin cause bone loss and the formation of granulation tissue. Endotoxin is believed to be involved in their pathogenesis. When the offending tooth is successfully treated, regeneration occurs resulting in the replacement of granulation tissue by bone. This process is likely to involve the migration of osteoblastic cells to the lesion and the subsequent formation of bone matrix: The potential role of Platelet-Derived Growth Factors (PDGF), a major cytokin involved in wound healing, in stimulating the direct migration of osteoblastic cells has been investigated. Furthermore, the effect of endotoxin on the human osteoblasts migration induced by PDGF has been tested directly and indirectly by means of interleukin-l[beta]. The results of this in vitro study show that: (1) Human bone cells migrate in a dose-dependent response when exposed to a gradient of Platelet Derived Growth Factor and that the maximum migration effect occurs at a concentration of 10 ng/ml of PDGF-AA or PDGF-BB; (2) the direct effect of endotoxin on osteoblastic cell migration revealed no conclusive pattern; however, (3) when normal human osteoblastic cells were pretreated with interleukin-l[beta] and then assayed for PDGF' induced cell-migration, a decrease of up to 42 % was observed for PDGF-AA. This reduction was statistically significant (p<0.01). For PDGF-BB, pretreament with Il-1[beta] induced a 23 % reduction in cell migration, but this was not statistically significant (p>O.01). These results suggest strongly the implication of endotoxin mediated by interleukin-1[beta] in the pathogenesis of lesions of endodontic origin.
Description
Thesis (D.Sc.D)--Boston University, Henry M. Goldman School of Graduate Dentistry, 1991 (Endodontics).
Includes bibliographical references (leaves 165-182).
Includes bibliographical references (leaves 165-182).
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This work is being made available in OpenBU by permission of its author, and is available for research purposes only. All rights are reserved to the author.