Improved isolation of anaerobic bacteria from the human gingival crevice by maintaining continuous anaerobiosis
Date
1969
DOI
Authors
Stutman, Marvin
Version
OA Version
Citation
Abstract
Using conventional aerobic and anaerobic culturing techniques only a portion on those microorganisms present in the gingival crevice of man have been cultivated. Since bacteria in this area appear to play an important role in dental and periodontal inflammatory disease, their isolation and characterization. A roll tube technique (Hungate method) was employed in an attempt to cultivate a maximal portion of the organisms of the gingival crevice area. This technique achieves an anaerobic state by flushing the local environment with oxygen free gas. Once collected the crevicular debris was immediately placed into sterile oxygen free test tubes which were flushed out by the oxygen free gas. In this manner the sample was weighed, dispersed, diluted and "plated" in roll tubes and plates. The media for control (Brewer jar technique) and Hungate techniques was heart fusion agar (Difco) fortified with ten percent defibrinated horse blood. Results indicated that when the Hungate technique was used, the recovery of viable bacteria, as a percentage of the total count was significantly greater than plates incubated aerobically or utilizing the Brewer anaerobic technique. Hungate percent recovery averaged forty-eight percent or ten samples when ninety percent nitrogen,ten percent hydrogen was used; sixty-seven percent for eight samples when eighty-five percent nitrogen, ten percent hydrogen, five percent carbon dioxide was used and sixty-three percent for eight samples when one hundred percent carbon dioxide was the gaseous atmosphere. At no time did the average for the anaerobic plates (Brewer jar) yield more than twenty six percent. This suggests that exclusion of oxygen and the presence of carbon dioxide maximize recovery of gingival crevice bacteria.
Description
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Thesis (M.Sc.D.)--Boston University School of Graduate Dentistry, 1969.
Bibliography, tables included.
Thesis (M.Sc.D.)--Boston University School of Graduate Dentistry, 1969.
Bibliography, tables included.
License
This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.