The effects of age and sex as factors in determining the efficacy of naringenin on dental caries and periodontal disease in the rat
Date
1986
DOI
Authors
Al-Sayegh, Hassan H
Version
OA Version
Citation
Abstract
The purpose of this study was to determine the interaction of age and sex on the efficacy of naringenin on dental caries and periodontal disease.
A total of sixty four (64) albino rats of both sexes (21-23 days of age) were used. The male and female controls were fed a basal cariogenic diet (50% sucrose), and compared to males and females fed the same diet but supplemented with naringenin (at a level of 0.7%).
At the end of twenty eight (28) days, eight male and eight female rats from each dietary group were sacrificed. The remaining animals were continued on the diets for an additional twenty eight (28) days. The rats were examined for dental caries and alveolar bone loss. Tooth surfaces were examined under a dissecting microscope for carious lesions; alveolar bone loss was scored on the buccal and lingual surfaces, by using a scaling lens.
The results showed that naringenin had little or no inhibitory effect on dental caries. The extent of the carious lesions seemed to be confined not only to enamel, but penetrated dentin and sometimes the pulpal chamber itself. The results indicated that there is a symmetrical distribution in the carious lesions between the jaws. Also, there was no significant difference in dental caries between males and females. Concerning the effect of age on dental caries, it was found that the older animals developed higher incidence of dental caries than did the prepubertal rats. Also it was noted that there is a correlation between body weight and the severity of dental lesions.
From the alveolar bone loss scoring, naringenin has little or no inhibitory effect. The lingual surfaces of the mandibular molars showed the highest bone loss, while the buccal surfaces of the same teeth showed the least amount of bone resorption. Unlike dental caries, age and sex were found to be significant factors in determining the amount of the alveolar bone loss in the maxillary and mandibular areas. Furthermore, these results indicated an age and sex interaction. Naringenin supplementation had no effect on body weight responses.
In summary, naringenin was found to have no inhibitory effects on dental caries or periodontal disease (alveolar bone loss). The cariogenic diet selected produced tooth caries and periodontal disease in male and female rats. Both age and sex are significant factors for determining the amount of alveolar bone loss, while age is the only significant factor for determining the degree of the dental caries. A milder cariogenic diet is recommended for future studies, so as not to mask any possible effects of naringenin.
Description
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Colored photographs included.
Thesis (M.Sc.D.)--Boston University, Henry M. Goldman School of Graduate Dentistry, 1986 (Pediatric Dentistry)
Bibliography : leaves 54-71.
Colored photographs included.
Thesis (M.Sc.D.)--Boston University, Henry M. Goldman School of Graduate Dentistry, 1986 (Pediatric Dentistry)
Bibliography : leaves 54-71.
License
This work is protected by copyright. Downloading is restricted to the BU community. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.