Microbial biosensors in whole blood: characterization towards at-home fertility hormone monitoring
Embargo Date
2027-01-28
OA Version
Citation
Abstract
Fertility hormone testing provides vital information for women undergoing In Vitro Fertilization (IVF), ectopic pregnancies, miscarriages, and polycystic ovary syndrome (PCOS). The current methods of testing pose many accessibility barriers to women around the world. At-home urine tests lack the sensitivity and specificity of blood tests that healthcare providers desire. However, blood tests require the patient to rely on testing centers where sample preparation is required, and results can take days. My research aims to use genetically engineered microbial cells as a robust sensing platform for quantifiabledetection of fertility hormones in whole blood. With a device using this system, patients could conveniently monitor their hormone levels from the comfort of their own home. However, the function of microbial cells in blood has not yet been established. In order to test both the survivability and sensing ability of engineered cells in blood, we synthesized four variants that would fluoresce in the presence of a model target molecule. The goal of this thesis is to characterize the function of microbial sensors in whole blood. Our experiments revealed successful reporter action, however fluorescence signal is lower in blood than in standard media conditions. We found that this could be because of lower growth in blood than expected. These results inform the development of our fertility hormone sensors and break down barriers to reproductive healthcare.
Description
2025