Investigating the role of TRPV4 and Pannexin1 in calcium signaling during corneal wound healing
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Abstract
Corneal wound healing is unique in that it relies on calcium signaling because it is avascular. This study looks at different aspects of corneal wound healing and the associated calcium signaling to determine the role of TRPV4 and Pannexin1. Both are involved in corneal healing. The study used in-vitro and ex-vivo conditions. In-vitro conditions used Human Corneal Limbal Epithelial (HCLE) cells. The cells were incubated with a combination of TRPV4 inhibitor and Pannexin1 inhibitor. The cells were imaged using a confocal microscope. The in-vitro conditions were repeated in ex-vivo conditions, and 10-week-old mice eyes were used. Matlab, Image J, and Microsoft Excel software converted the videos to quantifiable data. Increased or decreased calcium signaling was analyzed, indicating changes in or between cells. Experimental conditions were compared to control to determine significance. The results showed a significant decrease in calcium signaling after incubation with a TRPV4 and Pannexin1 inhibitor. The combination of Pannexin1 and TRPV4 inhibition showed potential interactions, and support for this interaction is seen mainly in histograms and hierarchical graphs but also in mean and total signaling analysis. TRPV4 and Pannexin1 are involved in corneal healing.Pannexin1 and TRPV4 could likely mediate each other, but further investigation must be done to determine this.
Description
2024