Multiwindow SRS imaging using a rapid widely tunable fiber laser
Files
Accepted manuscript
Date
2021-11-30
Authors
Ni, Hongli
Lin, Peng
Zhu, Yifan
Zhang, Meng
Tan, Yuying
Zhan, Yuewei
Wang, Zian
Cheng, Ji-Xin
Version
Accepted manuscript
OA Version
Citation
H. Ni, P. Lin, Y. Zhu, M. Zhang, Y. Tan, Y. Zhan, Z. Wang, J.-.X. Cheng. 2021. "Multiwindow SRS Imaging Using a Rapid Widely Tunable Fiber Laser.." Anal Chem, Volume 93, Issue 47, pp. 15703 - 15711. https://doi.org/10.1021/acs.analchem.1c03604
Abstract
Spectroscopic stimulated Raman scattering (SRS) imaging has become a useful tool finding a broad range of applications. Yet, wider adoption is hindered by the bulky and environmentally sensitive solid-state optical parametric oscillator (OPO) in a current SRS microscope. Moreover, chemically informative multiwindow SRS imaging across C-H, C-D, and fingerprint Raman regions is challenging due to the slow wavelength tuning speed of the solid-state OPO. In this work, we present a multiwindow SRS imaging system based on a compact and robust fiber laser with rapid and wide tuning capability. To address the relative intensity noise intrinsic to a fiber laser, we implemented autobalanced detection, which enhances the signal-to-noise ratio of stimulated Raman loss imaging by 23 times. We demonstrate high-quality SRS metabolic imaging of fungi, cancer cells, and Caenorhabditis elegans across the C-H, C-D, and fingerprint Raman windows. Our results showcase the potential of the compact multiwindow SRS system for a broad range of applications.