Design of a high-resolution light field miniscope for volumetric imaging in scattering tissue
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Published version
Date
2020
DOI
Authors
Chen, Yanqin
Xiong, Bo
Xue, Yujia
Jin, Xin
Greene, Joseph
Tian, Lei
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OA Version
Published version
Citation
Yanqin Chen, Bo Xiong, Yujia Xue, Xin Jin, Joseph Greene, Lei Tian. 2020. "Design of a high-resolution light field miniscope for volumetric imaging in scattering tissue." Biomedical Optics Express, Volume 11, pp. 1662 - 1678.
Abstract
Integrating light field microscopy techniques with existing miniscope architectures
has allowed for volumetric imaging of targeted brain regions in freely moving animals. However,
the current design of light field miniscopes is limited by non-uniform resolution and long
imaging path length. In an effort to overcome these limitations, this paper proposes an optimized
Galilean-mode light field miniscope (Gali-MiniLFM), which achieves a more consistent resolution
and a significantly shorter imaging path than its conventional counterparts. In addition, this
paper provides a novel framework that incorporates the anticipated aberrations of the proposed
Gali-MiniLFM into the point spread function (PSF) modeling. This more accurate PSF model
can then be used in 3D reconstruction algorithms to further improve the resolution of the platform.
Volumetric imaging in the brain necessitates the consideration of the effects of scattering. We
conduct Monte Carlo simulations to demonstrate the robustness of the proposed Gali-MiniLFM
for volumetric imaging in scattering tissue.
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© 2020 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.